Isolation of Pheophytin A and β-amyrin from Newbouldia laevis (P. Beauv) Leaf Extract

Pulverized leaves samples of Newbouldia laevis were extracted by cold maceration using methanol. The extract was concentrated in vacuo to yield a reddish brown solid 120.191 g. crude methanol partitioned into n-hexane 0.1 g, dichloromethane 2.5 ethyl acetate 4.6 and 10.0 fractions via coarse chromatography. Methanol fraction gave the highest subjected further purification repeated column chromatography pure components, namely NLM24 (R f 0.48), EAc:n-hex:MeOH (4:5:1) NLM19 0.47), EAc:n-hex:MeOH, respectively. These 1 H NMR, 13 C, COSY, HSQC HMBC spectroscopy. Pheophytin A β-amyrin proposed as structures isolated compounds. Even though not used for analgesic activity, literature reveals that pheophytin & are potent analgesics.